| Prefect: |   Cardinal Luis Antonio Gokim Tagle (67) (2019.12.08 – 2022.06.05) |
| Prefect: |   Cardinal Fernando Filoni (斐洛尼) (78) (2011.05.10 – 2019.12.08) |
| Prefect: |  Cardinal Ivan Dias (2006.05.20 – 2011.05.10) |
| Prefect: |  Cardinal Crescenzio Sepe (81) (2001.04.09 – 2006.05.20) |
| Prefect: |  Cardinal Jozef Tomko (1985.05.27 – 2001.04.09) |
| Prefect: |  Cardinal Agnelo Rossi (1970.10.22 – 1984.04.08) |
| Prefect: |  Cardinal Krikor Bédros XV Agagianian (1960.07.18 – 1970.10.19) |
| Prefect: | Cardinal Pietro Fumasoni Biondi (1933.03.16 – 1960.07.12) |
| Prefect: |  Cardinal Wilhelmus Marinus van Rossum, C.SS.R. (1918.03.12 – 1932.08.30) |
| Prefect: | Cardinal Domenico Serafini, O.S.B. Subl. (1916.03.24 – 1918.03.05) |
| Prefect: | Cardinal Girolamo Maria Gotti, O.C.D. (1902.07.29 – 1916.03.19) |
| Prefect: |  Cardinal Mieczysław Halka Ledóchowski (1892.01.26 – 1902.07.22) |
| Prefect: | Cardinal Giovanni Simeoni (1878.03.05 – 1892.01.14) |
| Prefect: | Cardinal Alessandro Franchi (1874.03.10 – 1878.03.05) |
| Prefect: | Cardinal Alessandro Barnabó (1856.06.20 – 1874.02.24) |
| Prefect: | Cardinal Giacomo Filippo Fransoni (1834.11.21 – 1856.04.20) |
| Prefect: | Cardinal Carlo Maria Pedicini (1831.02.04 – 1834.12.19) |
| Prefect: | Cardinal Bartolomeo Alberto Cappellari, O.S.B. Cam. (later Pope Gregory XVI) (1826.10.01 – 1831.02.02) |
| Prefect: | Cardinal Ercole Consalvi (1824.01.13 – 1824.01.24) |
| Prefect: | Cardinal Francesco Luigi Fontana, B. (1818.09.24 – 1822.03.19) |
| Prefect: | Cardinal Lorenzo Litta (1814.05.20 – 1818.09.24) |
| Prefect: | Cardinal Michele Di Pietro (1805.05.24 – 1814.05.20) |
| Prefect: | Cardinal Stefano Borgia (1802.08.16 – 1804.11.23) |
| Prefect: |  Cardinal Hyacinthe Sigismond Gerdil, B. (1795.02.27 – 1802.08.12) |
| Prefect: | Cardinal Leonardo Antonelli (1780.05.02 – 1795.02.27) |
| Prefect: | Cardinal Giuseppe Maria Castelli (1763.04.26 – 1780.04.09) |
| Prefect: | Cardinal Giuseppe Spinelli (1756.09.11 – 1763.04.12) |
| Prefect: | Cardinal Silvio Valenti Gonzaga (1747.03.27 – 1756.08.28) |
| Prefect: | Cardinal Vincenzo Petra (1727.01.10 – 1747.03.21) |
| Prefect: | Cardinal Giuseppe Sacripante (1704.12.09 – 1727.01.04) |
| Prefect: | Cardinal Carlo Barberini (1698.07.17 – 1704.10.02) |
| Prefect: | Cardinal Paluzzo Paluzzi Altieri degli Albertoni (1671.08.02 – 1698.06.29) |
| Prefect: |  Cardinal Antonio Barberini, S.M.O.M. (1632.12 – 1671.08.03) |
| Prefect: | Cardinal Ludovico Ludovisi (1622 – 1632) |
| Pro-Prefect: | Cardinal Jozef Tomko (1985.04.24 – 1985.05.27) |
| Pro-Prefect: |  Archbishop Dermot J. Ryan (1984.04.08 – 1985.02.21) |
| Pro-Prefect: | Cardinal Krikor Bédros XV Agagianian (1958.06.18 – 1960.07.18) |
| Pro-Prefect: |  Cardinal Samuel Alphonse Stritch (1958.03.01 – 1958.05.26) |
| Pro-Prefect: | Cardinal Domenico Serafini, O.S.B. Subl. (1916.02.26 – 1916.03.24) |
| Pro-Prefect: | Cardinal Giulio Maria Della Somaglia (1824.01.23 – 1826.10.01) |
| Pro-Prefect: | Cardinal Ercole Consalvi (1822.03.23 – 1824.01.13) |
| Pro-Prefect: | Cardinal Antonio Dugnani (1804.10.31 – 1805.05.16) |
| Pro-Prefect: | Cardinal Stefano Borgia (1798.05.25 – 1800.09.27) |
| Vice-Prefect: | Cardinal Luigi Capponi (1645.10.27 – 1653) |
| Secretary: |  Archbishop Protase Rugambwa (64) (later Cardinal) (2017.11.09 – 2022.06.05) |
| Secretary: |   Archbishop Savio Hon Tai-fai (韓大輝), S.D.B. (73) (2010.12.23 – 2017.09.28) |
| Secretary: |  Archbishop Robert Sarah (79) (later Cardinal) (2001.10.01 – 2010.10.07) |
| Secretary: | Archbishop Marcello Zago, O.M.I. (1998.03.28 – 2001.03.01) |
| Secretary: |  Archbishop Josip Uhač (1991.06.21 – 1998.01.18) |
| Secretary: | Archbishop José Tomás Sánchez (later Cardinal) (1985.10.30 – 1991.06.21) |
| Secretary: | Cardinal Duraisamy Simon Lourdusamy (1973.02.26 – 1985.10.30) |
| Secretary: |  Archbishop Bernardin Gantin (later Cardinal) (1973.02.26 – 1975.12.19) |
| Secretary: | Archbishop Sergio Pignedoli (later Cardinal) (1967.06.10 – 1973.02.26) |
| Secretary: | Archbishop Pietro Sigismondi (1954 – 1967.05.25) |
| Secretary: | Archbishop Filippo Bernardini (1953.01.15 – 1954.08.26) |
| Secretary: | Archbishop Celso Benigno Luigi Costantini (剛恆毅) (later Cardinal) (1935.12.20 – 1953.01.12) |
| Secretary: | Archbishop Carlo Salotti (later Cardinal) (1930.07.03 – 1935.12.16) |
| Secretary: | Archbishop Francesco Marchetti Selvaggiani (later Cardinal) (1922.12.15 – 1930.06.30) |
| Secretary: | Archbishop Pietro Fumasoni Biondi (later Cardinal) (1921.06.14 – 1922.12.14) |
| Secretary: | Msgr. Camillo Laurenti (later Cardinal) (1911.08.12 – 1921.06.13) |
| Secretary: | Cardinal Agostino Ciasca, O.E.S.A. (1893.06.19 – 1902.02.06) |
| Secretary: | Archbishop Andrea Aiuti (later Cardinal) (1891.07.24 – 1893.06.07) |
| Secretary: | Cardinal Ignazio Persico (德斯馬曾), O.F.M. Cap. (1891.06.13 – 1893.05.30) |
| Secretary: | Archbishop Domenico Maria Jacobini (later Cardinal) (1882.03.30 – 1891.06.16) |
| Secretary: | Msgr. Mariano Rampolla del Tindaro (later Cardinal) (1880 – 1882.12.01?) |
| Secretary: | Msgr. Annibale Capalti (later Cardinal) (1861.03.30 – 1868.03.13) |
| Secretary: | Archbishop Gaetano Bedini (later Cardinal) (1856.06.20 – 1861.03.18) |
| Secretary: | Msgr. Castruccio Castracane degli Antelminelli (later Cardinal) (1828 – 1833.04.15) |
| Secretary: | Cardinal Pietro Caprano (1823.03.11 – 1829.04.04) |
| Secretary: | Msgr. Carlo Maria Pedicini (later Cardinal) (1816.07 – 1823.03.10) |
| Secretary: | Archbishop Cesare Brancadoro (later Cardinal) (1797.06.07 – 1800.08.11) |
| Secretary: | Archbishop Antonio Felice Zondadari (later Cardinal) (1789 – 1795) |
| Secretary: | Msgr. Stefano Borgia (later Cardinal) (1770 – 1789.03.30) |
| Secretary: | Msgr. Bartolomeo Ruspoli (later Cardinal) (1724 – 1728) |
| Secretary: | Archbishop Federico Baldeschi Colonna (later Cardinal) (1668.05.07 – 1673.03.22) |
| Pro-Secretary: | Archbishop Agostino Ciasca, O.E.S.A. (later Cardinal) (1892.09.19 – 1893.06.19) |
| Pro-Secretary: | Archbishop Antonio Felice Zondadari (later Cardinal) (1795 – 1801) |
| Pro-Secretary: | Msgr. Domenico Silvio Passionei (later Cardinal) (1720.11 – 1721.01) |
| Adjunct Secretary: | Archbishop Giovanni Pietro Dal Toso (59) (2017.11.09 – 2022.06.05) |
| Adjunct Secretary: | Archbishop Protase Rugambwa (64) (later Cardinal) (2012.06.26 – 2017.11.09) |
| Adjunct Secretary: |  Archbishop Piergiuseppe Vacchelli (87) (2008.05.24 – 2012.06.26) |
| Adjunct Secretary: |  Archbishop Henryk Hoser, S.A.C. (2005.01.22 – 2008.05.24) |
| Adjunct Secretary: |  Archbishop Albert Malcolm Ranjith Patabendige Don (76) (later Cardinal) (2001.10.01 – 2005.12.10) |
| Adjunct Secretary: | Archbishop Charles Asa Schleck, C.S.C. (1995.02.10 – 2001.10.01) |
| Adjunct Secretary: | Archbishop Bernardin Gantin (later Cardinal) (1971.06.29 – 1973.02.26) |
| Undersecretary: | Fr. Ryszard Szmydki, O.M.I. (2017.09.28 – 2022.06.05) |
| Undersecretary: | Fr. Tadeusz Wojda, S.A.C. (67) (later Archbishop) (2012.07.24 – 2017.04.12) |
| Undersecretary: | Fr. Massimo Cenci, P.I.M.E. (2001.10.01 – 2012.05.11) |
| Undersecretary: | Msgr. Luigi Ghidoni (1995 – 2001) |
| Undersecretary: | Fr. Charles Asa Schleck, C.S.C. (later Archbishop) (1986 – 1995.02.10) |
| Undersecretary: | Msgr. Tiziano Scalzotto (1976 – 1986) |
| Undersecretary: | Msgr. Giovanni Battista Reghezza (1973 – 1976) |
| Undersecretary: | Msgr. Edoardo Pecoraio (later Archbishop) (1961 – 1971.12.28) |
| Undersecretary: | Msgr. Carlo Corvo (1952 – 1960) |
| Undersecretary: | Msgr. Giulio Chiavoni (? – 1952) |
| Undersecretary: | Msgr. Giovanni Brunelli (later Cardinal) (1834 – 1837) |
| Delegate of the Administration: |  Dr. Benjamín Estévez de Cominges (50) (2021.12.17 – 2022.06.05) |
| Delegate of the Administration: | Msgr. Angelo Mottola (later Archbishop) (1986 – 1999.07.16) |
(x 29)