| Prefect: |  Cardinal Luis Francisco Ladaria Ferrer, S.J. (81) (2017.07.01 – 2023.07.01) |
| Prefect: |  Cardinal Gerhard Ludwig Müller (77) (2012.07.02 – 2017.07.01) |
| Prefect: | Cardinal William Joseph Levada (2005.05.13 – 2012.07.02) |
| Prefect: | Cardinal Joseph Ratzinger (later Pope Benedict XVI) (1981.11.25 – 2005.04.02) |
| Prefect: | Cardinal Franjo Šeper (1968.08.20 – 1981.11.25) |
| Prefect: | Saint Pope John XXIII (1958.10.28 – 1963.06.03) |
| Prefect: | Pope Pius XII (1939.03.02 – 1958.10.09) |
| Prefect: | Pope Pius XI (1922.02.06 – 1939.02.10) |
| Prefect: | Pope Benedict XV (1914.09.03 – 1922.01.22) |
| Prefect: | Saint Pope Pius X (1903.08.04 – 1914.08.20) |
| Grand Inquisitor: | Cardinal Giulio Antonio Santorio (1587 – 1602.05.09) |
| Grand Inquisitor: | Cardinal Giacomo Savelli (1577 – 1587.12.05) |
| Grand Inquisitor: | Cardinal Scipione Rebiba (1573 – 1577.07.23) |
| Grand Inquisitor: | Cardinal Diego Espinosa y Arévalo (1566.09.08 – 1572.09.05) |
| Grand Inquisitor: | Cardinal Antonio-Michele Ghislieri, O.P. (later Pope Pius V, Saint) (1558 – 1566.01.07) |
| Grand Inquisitor: | Cardinal Gian Pietro Carafa (later Pope Paul IV) (1542.07.21 – 1555.05.23) |
| Pro-Prefect: | Cardinal Alfredo Ottaviani (1965 – 1968.01.06) |
| Assessor: | Archbishop Pietro Parente (later Cardinal) (1959.10.23 – 1965.12.07) |
| Assessor: | Archbishop Gabriel Acacius Coussa, B.A. (later Cardinal) (1953.01.15 – 1961.08.04) |
| Assessor: | Msgr. Alfredo Ottaviani (later Cardinal) (1935.12.19 – 1953.01.12) |
| Assessor: | Msgr. Nicola Canali (later Cardinal) (1926.06.27 – 1935.12.16) |
| Assessor: | Msgr. Carlo Perosi (later Cardinal) (1916.12.08 – 1926.06.21) |
| Assessor: | Archbishop Donato Sbarretti (later Cardinal) (1914.06.08 – 1916.12.04) |
| Assessor: | Archbishop Domenico Serafini, O.S.B. Subl. (later Cardinal) (1911.11.30 – 1914.05.25) |
| Assessor: | Msgr. Giovanni Battista Lugari (later Cardinal) (1902.01.11 – 1911.11.27) |
| Assessor: | Archbishop Casimiro Gennari (later Cardinal) (1895.11.15 – 1901.04.15) |
| Assessor: | Msgr. Francesco Segna (later Cardinal) (1893.06.20 – 1894.05.18) |
| Assessor: | Msgr. Raffaele Monaco La Valletta (later Cardinal) (1859.01.25 – 1868.03.13) |
| Assessor: | Cardinal Raffaele Mazio (1824.12.28 – 1832.02.04) |
| Secretary: |  Archbishop Giacomo Morandi (60) (2017.07.18 – 2022.01.10) |
| Secretary: |  Archbishop Luis Francisco Ladaria Ferrer, S.J. (81) (later Cardinal) (2008.07.09 – 2017.07.01) |
| Secretary: | Archbishop Angelo Amato, S.D.B. (later Cardinal) (2002.12.19 – 2008.07.09) |
| Secretary: |  Archbishop Tarcisio Bertone, S.D.B. (90) (later Cardinal) (1995.06.13 – 2002.12.10) |
| Secretary: | Archbishop Alberto Bovone (later Cardinal) (1984.04.08 – 1995.06.13) |
| Secretary: | Archbishop Jean Jérôme Hamer, O.P. (later Cardinal) (1973.06.14 – 1984.04.08) |
| Secretary: | Cardinal Paul-Pierre Philippe, O.P. (1967.06.29 – 1973.03.06) |
| Secretary: | Archbishop Pietro Parente (later Cardinal) (1965.12.07 – 1967.06.26) |
| Secretary: | Cardinal Alfredo Ottaviani (1959.11.07 – 1965) |
| Secretary: | Cardinal Giuseppe Pizzardo (1951.02.16 – 1959.10.12) |
| Secretary: | Cardinal Francesco Marchetti Selvaggiani (1939.04.30 – 1951.01.13) |
| Secretary: | Cardinal Donato Sbarretti (1930.07.04 – 1939.04.01) |
| Secretary: | Cardinal Rafael Merry del Val y Zulueta (1914.10.14 – 1930.02.26) |
| Secretary: | Cardinal Domenico Ferrata (1914.01.02 – 1914.10.10) |
| Secretary: | Cardinal Mariano Rampolla del Tindaro (1908.12.30 – 1913.12.16) |
| Secretary: | Cardinal Serafino Vannutelli (1903.01.16 – 1908.12.31) |
| Secretary: | Cardinal Lucido Maria Parocchi (1896 – 1903.01.15) |
| Secretary: | Cardinal Raffaele Monaco La Valletta (1884.02.15 – 1896.07.14) |
| Secretary: | Cardinal Luigi Bilio, B. (1883.01.25 – 1884.01.30) |
| Secretary: | Cardinal Antonio Maria Panebianco, O.F.M. Conv. (1882.03.30 – 1883.01.25) |
| Secretary: | Cardinal Prospero Caterini (1876.12.21 – 1881.10.28) |
| Secretary: | Cardinal Costantino Patrizi Naro (1860.10.10 – 1876.12.17) |
| Secretary: | Cardinal Vincenzo Macchi (1844.04.25 – 1860.09.30) |
| Secretary: | Cardinal Bartolomeo Pacca (1830.04.07 – 1844.04.19?) |
| Secretary: | Cardinal Giulio Maria Della Somaglia (1814.05.20 – 1830.04.02) |
| Secretary: | Cardinal Leonardo Antonelli (1800.11.08 – 1811.01.23) |
| Secretary: | Cardinal Carlo Rezzonico (1777.01.17 – 1799.01.26) |
| Secretary: | Cardinal Luigi Maria Torrigiani (1775.02.22 – 1777.01.06) |
| Secretary: | Cardinal Giovanni Francesco Stoppani (1770.12.12 – 1774.11.18) |
| Secretary: | Cardinal Neri Maria Corsini (1753 – 1770.12.06?) |
| Secretary: | Cardinal Tommaso Ruffo (1740.08.29 – 1753.02.16) |
| Secretary: | Cardinal Pietro Ottoboni (1726.06.14 – 1740.02.29) |
| Secretary: | Cardinal Fabrizio Paolucci (1725 – 1726.06.12) |
| Secretary: | Cardinal Francesco del Giudice (1719 – 1725.10.10) |
| Secretary: | Cardinal Nicolò Acciaiuoli (1717 – 1719.02.23) |
| Secretary: | Cardinal Fabrizio Spada (1716 – 1717.06.15) |
| Secretary: | Cardinal Galeazzo Marescotti (1700 – 1716) |
| Secretary: | Cardinal Alderano Cibo-Malaspina (1683 – 1700.07.22) |
| Secretary: | Cardinal Cesare Facchinetti (1679 – 1683.01.30) |
| Secretary: | Cardinal Francesco Barberini (1633 – 1679.12.10) |
| Secretary: | Cardinal Antonio Marcello Barberini, O.F.M. Cap. (1629 – 1633) |
| Secretary: | Cardinal Giovanni Garzia Millini (1616 – 1629.10.02) |
| Secretary: | Cardinal Pompeio Arrigoni (1605 – 1616.04.04) |
| Secretary: | Cardinal Camillo Borghese (later Pope Paul V) (1602 – 1605.05.16) |
| Pro-Secretary: | Cardinal Alfredo Ottaviani (1953.01.15 – 1959.11.07) |
| Adjunct Secretary: |  Archbishop Joseph Augustine Di Noia, O.P. (82) (2013.09.21 – 2023.07.10) |
| Undersecretary: | Msgr. Matteo Visioli (2017.09.14 – 2022.07.06) |
| Undersecretary: |  Msgr. Giacomo Morandi (60) (later Archbishop) (2015.10.27 – 2017.07.18) |
| Undersecretary: | Msgr. Damiano Marzotto Caotorta (2009.06.20 – 2015) |
| Undersecretary: |  Fr. Joseph Augustine Di Noia, O.P. (82) (later Archbishop) (2002.04.04 – 2009.06.16) |
| Undersecretary: |  Fr. Gianfranco Girotti, O.F.M. Conv. (88) (later Bishop) (1997.06.11 – 2002.02.16) |
| Undersecretary: | Msgr. Jozef Zlatňanský (later Bishop) (1984.12.06 – 1997.06.11) |
| Undersecretary: | Archbishop Alberto Bovone (later Cardinal) (1973.05.21 – 1984.05.12) |
| Undersecretary: | Msgr. Charles Moeller (1966 – 1973) |
| Adjunct Undersecretary: |  Msgr. Armando Matteo (55) (2021.04.12 – 2022.04.23) |
| Promoter of Justice: | Msgr. Robert W. Oliver (2012.12.22 – 2014.09.10) |
| Promoter of Justice: |  Msgr. Charles Jude Scicluna (66) (later Archbishop) (2002.10.21 – 2012.10.06) |
| Promoter of Justice: | Fr. Marino Maccarelli, O.S.M. (1983 – 1995) |
| Promoter of Justice: | Msgr. Sándor Csertõ (1969 – 1982) |
| Promoter of Justice: | Msgr. Giuseppe Graneris (1952 – 1968) |
| Promoter of Justice: | Msgr. Giosuè Venturi (1950 – 1952) |
| Promoter of Justice: | Msgr. Vigilio Dalpiaz (1937 – 1950) |
| Promoter of Justice: | Msgr. Giuseppe Latini (1919 – 1937) |
| Adjunct Promoter of Justice: | Msgr. Sebastiano Masala (1965 – 1968.12.09) |
| Commissary: | Fr. Angelo Raimondo Verardo, O.P. (later Bishop) (1959 – 1965) |
| Commissary: | Fr. Paul-Pierre Philippe, O.P. (later Cardinal) (1955 – 1959.12.14) |
| Commissary: | Fr. Cristoforo Bigazzi, O.P. (1951 – 1955) |
| Commissary: | Fr. Giovanni Lottini, O.P. (1919 – 1951) |
| Commissary: | Fr. Domenico Pasqualigo, O.P. (1911? – 1919) |
| Commissary: | Archbishop Tommaso Maria Granello, O.P. (1897.10.02 – 1911.03.24) |
| Commissary: | Archbishop Vincenzo Leone Sallua, O.P. (1877.03.12 – 1896.12.21) |
| Vice-Commissary: | Fr. Domenico Pasqualigo, O.P. (1905 – 1911?) |
| Bureau Chief: | Msgr. Alejandro Cifres Giménez (2013.12.04 – 2021.07.28) |
| Bureau Chief: | Fr. Hermann Geissler, F.S.O. (2009.08.04 – 2019.01.30) |
| Bureau Chief: | Fr. Pedro Miguel Funes Díaz (2009.01.31 – 2017.04.05) |
| Bureau Chief: | Msgr. Damiano Marzotto Caotorta (2003.02.13 – 2009.06.20) |
| Bureau Chief: |  Msgr. Raffaello Martinelli (77) (later Bishop) (1999.01.04 – 2009.07.02) |