| Cardinal-Bishops (6) |
| 1 |  | Luigi Micara, O.F.M. Cap. (70), Cardinal-Bishop of Ostia–Velletri, Prefect of Sacred Congregation of Ceremonies and Cardinal Dean of Sacred College of Cardinals |
| 2 | | Vincenzo Macchi (75), Cardinal-Bishop of Porto e Santa Rufina, Secretary of Commission of Roman and Universal Inquisition and Cardinal Vice-Dean of Sacred College of Cardinals |
| 3 | | Luigi Lambruschini, B. (70), Cardinal-Bishop of Sabina, Prefect of Sacred Congregation of Studies, Librarian of Vatican Apostolic Library and Archivist of Vatican Secret Archives |
| 4 | | Pietro Ostini (71), Cardinal-Bishop of Albano and Prefect of Sacred Congregation of Bishops and Regulars |
| 5 | | Castruccio Castracane degli Antelminelli (66), Cardinal-Bishop of Palestrina, Major Penitentiary of Sacred Apostolic Penitentiary and Camerlengo of Sacred College of Cardinals |
| 6 | | Mario Mattei (53), Cardinal-Bishop of Frascati, President of Sacred Congregation of the Reverend Basilica of Saint Peter and Archpriest of Papal Basilica of Saint Peter |
| Cardinal-Priests (36) |
| 7 | | Carlo Oppizzoni (77), Cardinal-Priest of S. Lorenzo in Lucina, Metropolitan Archbishop of Bologna (Italy) and Protopriest of Sacred College of Cardinals |
| 8 | | Giacomo Filippo Fransoni (70), Cardinal-Priest of S. Maria in Ara Coeli and Prefect of Sacred Congregation of the Propagation of the Faith |
| 9 | | Benedetto Colonna Barberini di Sciarra (57), Cardinal-Priest of S. Maria in Trastevere and Archpriest of Papal Archbasilica of Saint John Lateran |
| 10 | | Francesco Serra-Casano (63), Cardinal-Priest of Ss. XII Apostoli and Metropolitan Archbishop of Capua (Italy) |
| 11 | | Ugo Pietro Spinola (54), Cardinal-Priest of S. Martino ai Monti, Pro-Datary of His Holiness of Apostolic Dataria and Commendatory Abbot emeritus of Subiaco (Italy) |
| 12 | | Giacomo Luigi Brignole (49), Cardinal-Priest of S. Cecilia, Cardinal-Priest of S. Giovanni a Porta Latina in commendum, Titular Archbishop emeritus of Nazianzus and Treasurer General emeritus of Reverend Apostolic Camera |
| 13 | | Paolo Polidori (68), Cardinal-Priest of S. Prassede, Commendatory Abbot of Subiaco (Italy) and Prefect of Sacred Congregation of the Council |
| 14 | | Costantino Patrizi Naro (47), Cardinal-Priest of S. Silvestro in Capite, Archpriest of Papal Basilica of Saint Mary Major and Vicar General for the Vicariate of Rome of Roma (Italy) |
| 15 | | Giuseppe Alberghini (75), Cardinal-Priest of S. Prisca |
| 16 | | Ambrogio Bianchi, O.S.B. Cam. (74), Cardinal-Priest of Ss. Andrea e Gregorio al Monte Celio and Prior General of Camaldolese Congregation of the Order of Saint Benedict (Camaldolese) |
| 17 | | Gabriel Della Genga Sermattei (44), Cardinal-Priest of S. Girolamo dei Croati and Metropolitan Archbishop emeritus of Ferrara (Italy) |
| 18 | | Luigi Amat di San Filippo e Sorso (49), Cardinal-Priest of S. Maria in Via |
| 19 | | Angelo Mai (64), Cardinal-Priest of S. Anastasia and Prefect of Sacred Congregation of the Index |
| 20 | | Giovanni Soglia Ceroni (66), Cardinal-Priest of Ss. Quattro Coronati, Archbishop-Bishop of Osimo (Italy) and Archbishop-Bishop of Cingoli (Italy) |
| 21 | | Chiarissimo Falconieri Mellini (51), Cardinal-Priest of S. Marcello and Metropolitan Archbishop of Ravenna (Italy) |
| 22 | | Antonio Francesco Orioli, O.F.M. Conv. (67), Cardinal-Priest of S. Maria sopra Minerva and Bishop emeritus of Orvieto (Italy) |
| 23 | | Antonio Tosti (69), Cardinal-Priest of S. Pietro in Montorio and Treasurer General emeritus of Reverend Apostolic Camera |
| 24 | | Giuseppe Gasparo Mezzofanti (71), Cardinal-Priest of S. Onofrio and Prefect of Sacred Congregation of Studies |
| 25 | | Filippo De Angelis (54), Cardinal-Priest of S. Bernardo alle Terme and Metropolitan Archbishop of Fermo (Italy) |
| 26 | | Gabriele Ferretti (51), Cardinal-Priest of Ss. Quirico e Giulitta, Prefect of Sacred Congregation of Indulgences and Sacred Relics and Metropolitan Archbishop emeritus of Fermo (Italy) |
| 27 | | Carlo Januarius Acton (43), Cardinal-Priest of S. Maria della Pace and Cardinal Protector of Pontifical Ecclesiastical Academy |
| 28 | | Ferdinando Maria Pignatelli, C.R. (76), Cardinal-Priest of S. Maria della Vittoria and Metropolitan Archbishop of Palermo (Italy) |
| 29 | | Giovanni Maria Mastai Ferretti (54), Cardinal-Priest of Ss. Marcellino e Pietro and Archbishop-Bishop of Imola (Italy) |
| 30 | | Gaspare Bernardo Pianetti (66), Cardinal-Priest of S. Sisto, Bishop of Viterbo (Italy) and Bishop of Tuscanella (Italy) |
| 31 | | Luigi Vannicelli Casoni (45), Cardinal-Priest of S. Callisto and Vice-Camerlengo of the Holy Roman Church emeritus of Reverend Apostolic Camera |
| 32 | | Lodovico Altieri (40), Cardinal-Priest of S. Maria in Portico pro hac vice Title |
| 33 | | Tommaso Pasquale Gizzi (58), Cardinal-Priest of S. Pudenziana |
| 34 | | Cosimo Corsi (48), Cardinal-Priest of Ss. Giovanni e Paolo and Bishop of Jesi (Italy) |
| 35 | | Antonio Maria Cadolini, B. (74), Cardinal-Priest of S. Clemente and Bishop of Ancona e Umana (Italy) |
| 36 | | Fabio Maria Asquini (43), Cardinal-Priest of S. Stefano al Monte Celio and Patriarch emeritus of Constantinople |
| 37 | | Antonio Maria Cagiano de Azevedo (48), Cardinal-Priest of S. Croce in Gerusalemme and Bishop of Senigallia (Italy) |
| 38 | | Niccola Clarelli Parracciani (47), Cardinal-Priest of S. Pietro in Vincoli, Bishop of Corneto (Italy) and Bishop of Montefiascone (Italy) |
| 39 | | Domenico Carafa della Spina di Traetto (40), Cardinal-Priest of S. Maria degli Angeli and Metropolitan Archbishop of Benevento (Italy) |
| 40 | | Lorenzo Simonetti (57), Cardinal-Priest of S. Lorenzo in Panisperna |
| 41 | | Giacomo Piccolomini (50), Cardinal-Priest of S. Balbina |
| 42 | | Sisto Riario Sforza (35), Cardinal-Priest of S. Sabina and Metropolitan Archbishop of Napoli (Italy) |
| Cardinal-Deacons (8) |
| 43 | | Tommaso Riario Sforza (64), Cardinal-Deacon of S. Maria in Via Lata, Camerlengo of the Holy Roman Church of Reverend Apostolic Camera and Protodeacon of Sacred College of Cardinals |
| 44 | | Tommaso Bernetti (66), Cardinal-Deacon of S. Lorenzo in Damaso pro illa vice Deaconry and Vice-Chancellor of Apostolic Chancery |
| 45 | | Luigi Gazzoli (72), Cardinal-Deacon of S. Eustachio and Prefect of Sacred Congregation of Good Government |
| 46 | | Adriano Fieschi (58), Cardinal-Deacon of S. Maria ad Martyres and Prefect emeritus of Prefecture of the Holy Apostolic Palaces |
| 47 | | Luigi Ciacchi (80), Cardinal-Deacon of S. Angelo in Pescheria and Vice-Camerlengo of the Holy Roman Church emeritus of Reverend Apostolic Camera |
| 48 | | Giuseppe Ugolini (63), Cardinal-Deacon of S. Adriano al Foro |
| 49 |  | Francesco Saverio Massimo (40), Cardinal-Deacon of S. Maria in Domnica and Prefect emeritus of Prefecture of the Holy Apostolic Palaces |
| 50 | | Giovanni Serafini (59), Cardinal-Deacon of S. Maria in Cosmedin |
Francisco Javier de Cienfuegos y Jovellanos (70), Cardinal-Priest of S. Maria del Popolo, Metropolitan Archbishop of Sevilla (Spain) and Apostolic Administrator of Guadix–Baza (Spain)
Engelbert Sterckx (53), Cardinal-Priest of S. Bartolomeo all’Isola and Metropolitan Archbishop of Mechelen (Belgium)
Hugues-Robert-Jean-Charles de la Tour d’Auvergne-Lauraquais (77), Cardinal-Priest of S. Agnese fuori le mura and Bishop of Arras (France)
Friedrich Johannes Jacob Celestin von Schwarzenberg (37), Cardinal-Priest of S. Agostino and Metropolitan Archbishop of Salzburg (Austria)
Guilherme Henriques de Carvalho (53), Patriarch of Lisboa (Portugal), Bishop of Castelo Branco (Portugal) and Apostolic Administrator of Portalegre (Portugal)