| Date of Death | Deceased Cardinals (39) |
| 1600.01.21 | Cardinal-Priest of S. Maria in Traspontina (1596.12.02 – 1600.01.21) |
| 1600.01.21 | Cardinal-Priest of S. Sisto (1586.07.14 – 1600.01.21) |
| 1600.02.20 | Cardinal-Bishop of Porto e Santa Rufina (1591.03.20 – 1600.02.20) |
| 1600.04.20 | Cardinal-Bishop of Frascati (1600.02.21 – 1600.04.20) |
| 1600.08.27 | Cardinal-Bishop of Albano (1600.04.23 – 1600.08.27) |
| 1600.09.18 | Cardinal-Priest of Ss. XII Apostoli (1585.05.20 – 1600.09.18) Metropolitan Archbishop of Sevilla (Spain) (1581.10.20 – 1600.09.18) |
| 1600.11.12 | Cardinal-Deacon of S. Maria Nuova (1577.12.11 – 1600.11.12) |
| 1602.04.16 | Cardinal-Priest of S. Maria in Trastevere (1600.08.30 – 1602.04.16) Bishop emeritus of Saint-Papoul (France) (1561.08.08 – 1564) |
| 1602.05.09 | Cardinal-Bishop of Palestrina (1597.08.18 – 1602.05.09) Grand Inquisitor of Commission of Roman and Universal Inquisition (1587 – 1602.05.09) Major Penitentiary of Sacred Apostolic Penitentiary (1592.02.08 – 1602.05.09) |
| 1603.02.14 | Cardinal-Bishop of Ostia–Velletri (1591.03.20 – 1603.02.14) Metropolitan Archbishop of Napoli (Italy) (1596.02.12 – 1603.02.14) |
| 1603.06.14 | Cardinal-Bishop of Porto e Santa Rufina (1603.02.19 – 1603.06.14) Vicar General for the Vicariate of Rome emeritus of Roma (Italy) (1588 – 1603) |
| 1603.08.16 | Cardinal-Priest of S. Salvatore in Lauro (1599.03.17 – 1603.08.16) |
| 1603.09.01 | Cardinal-Priest of S. Biagio dell’Anello (1599.07.05 – 1603.09.01) Metropolitan Archbishop of Bari e Canosa (Italy) (1602.03.18 – 1603.09.01) |
| 1603.12.08 | Cardinal-Priest of S. Pancrazio (1592.03.09 – 1603.12.08) Commendatory Abbot of Nonantola (Italy) (1587 – 1603.12.08) Prefect of Sacred Congregation of the Council (1591.01.14 – 1603.12.08) |
| 1604.02.29 | Cardinal-Priest of Ss. Quirico e Giulitta (1593.10.11 – 1604.02.29) Bishop emeritus of Ripatransone (Italy) (1571.10.03 – 1575.05.20) Pro-Datary of His Holiness of Apostolic Dataria (1593 – 1604.02.29) |
| 1604.03.13 | Cardinal-Priest of S. Eusebio (1599.03.17 – 1604.03.13) |
| 1604.05.20 | Cardinal-Bishop of Sabina (1603.02.19 – 1604.05.20) |
| 1605.02.24 | Cardinal-Bishop of Porto e Santa Rufina (1603.06.16 – 1605.02.24) |
| 1605.04.27 | Cardinal-Priest of S. Pietro in Vincoli (1604.06.25 – 1605.04.27) |
| 1605.05.31 | Cardinal-Priest of S. Marcello (1599.03.17 – 1605.05.31) Bishop of Corneto (Italy) (1601.05.14 – 1605.05.31) Bishop of Montefiascone (Italy) (1601.05.14 – 1605.05.31) Prefect of Sacred Congregation of the Council (1604 – 1605.05.31) |
| 1605.07.16 | Cardinal-Priest of S. Clemente (1596.06.21 – 1605.07.16) |
| 1606.01.20 | Cardinal-Priest of S. Croce in Gerusalemme (1599.01.08 – 1606.01.20) |
| 1606.05.18 | Cardinal-Priest of Ss. Quattro Coronati (1592.03.04 – 1606.05.18) |
| 1606.05.23 | Cardinal-Bishop of Palestrina (1605.06.01 – 1606.05.23) |
| 1606.11.01 | Bishop emeritus of Amelia (Italy) (1562.10.09 – 1571) President of Sacred Congregation of the Reverend Basilica of Saint Peter (1580 – 1606.11.01) |
| 1607.02.03 | Cardinal-Bishop of Ostia–Velletri (1603.02.19 – 1607.02.03) Secretary of State emeritus of Apostolic Secretariat (1572 – 1585) |
| 1607.06.30 | Cardinal-Priest of Ss. Nereo ed Achilleo (1596.06.21 – 1607.06.30) Librarian of Vatican Apostolic Library (1597.05 – 1607.06.30) |
| 1607.08.17 | Cardinal-Priest of S. Pietro in Montorio (1604.06.24 – 1607.08.17) Metropolitan Archbishop of Chieti (Italy) (1607.02.12 – 1607.08.17) |
| 1607.11.24 | Cardinal-Deacon of S. Agata alla Suburra (1591.04.05 – 1607.11.24) Bishop of Strasbourg (France) (1604 – 1607.11.24) |
| 1608.01.19 | Cardinal-Priest of S. Giovanni a Porta Latina (1605.01.07 – 1608.01.19) Metropolitan Archbishop of Gniezno (Poland) (1606.07.31 – 1608.01.19) |
| 1608.05.17 | Cardinal-Bishop of Palestrina (1606.06.05 – 1608.05.17) Commendatory Abbot of Subiaco (Italy) (1592 – 1608.05.17) Archpriest of Papal Archbasilica of Saint John Lateran (1595 – 1608.05.17) |
| 1608.06.11 | Cardinal-Priest of S. Maria sopra Minerva (1602.06.17 – 1608.06.11) Metropolitan Archbishop emeritus of Siena (Italy) (1597.09.15 – 1607.01.24) |
| 1608.09.08 | Master of the Order emeritus of Order of Preachers (Dominicans) (1601.05.13 – 1607.12.10) |
| 1608.09.19 | Cardinal-Priest of S. Sisto (1600.01.24 – 1608.09.19) |
| 1609.01.08 | Cardinal-Priest of S. Martino ai Monti (1599.01.08 – 1609.01.08) Metropolitan Archbishop of Sevilla (Spain) (1601.04.30 – 1609.01.08) |
| 1609.01.11 | Cardinal-Priest of Ss. Marcellino e Pietro (1607.02.07 – 1609.01.11) Metropolitan Archbishop of Chieti (Italy) (1607.09.03 – 1609.01.11) |
| 1609.02.10 | Cardinal-Priest of S. Salvatore in Lauro (1604.06.25 – 1609.02.10) Patriarch emeritus of Alexandria (1602.08.26 – 1604.06.09) |
| 1609.02.22 | Cardinal-Deacon emeritus of S. Maria in Via Lata (1587.01.07 – 1588.11.28) Provost emeritus of Santo Stefano di Prato (Italy) (1574 – 1587) |
| 1609.07.08 | Cardinal-Priest of S. Pancrazio (1606.12.19 – 1609.07.08) Metropolitan Archbishop of Monreale (Italy) (1588.01.22 – 1609.07.08) Librarian of Vatican Apostolic Library (1607.07.04 – 1609.07.08) |
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