| Date of Death | Deceased Cardinals (39) |
| 1580.01.18 | Cardinal-Priest of S. Cesareo in Palatio pro hac vice Title (1570.07.03 – 1580.01.18) |
| 1580.01.31 | Cardinal-Priest of Ss. Quattro Coronati (1547.01.24 – 1580.01.31) Metropolitan Archbishop emeritus of Évora (Portugal) (1574.12.15 – 1578.07.04) |
| 1580.04.20 | Cardinal-Priest of S. Maria in Portico Octaviae pro hac vice Title (1569.05.13 – 1580.04.20) Bishop of Civitate (Italy) (1561.09.05 – 1580.04.20) Prefect of Sacred Congregation of the Council (1565.09 – 1580.04.20) |
| 1580.12.01 | Cardinal-Bishop of Ostia–Velletri (1570.07.03 – 1580.12.01) |
| 1580.12.23 | Bishop of Liège (Belgium) ([1564.06.05] 1565.02.23 – 1580.12.23) |
| 1581.05.16 | Cardinal-Priest of S. Prisca (1578.07.09 – 1581.05.16) Cardinal-Priest of S. Giovanni a Porta Latina in commendum (1565.11.17 – 1581.05.16) |
| 1581.05.16 | Cardinal-Priest of S. Maria in Via (1565.05.15 – 1581.05.16) Provost of Borgo San Donnino (Italy) (1562 – 1581.05.16) Bishop emeritus of Parma (Italy) (1560.04.26 – 1573.03.30) Archpriest of Papal Basilica of Saint Mary Major (1573? – 1581.05.16) |
| 1582.10.28 | Cardinal-Priest of S. Sabina (1579.08.03 – 1582.10.28) Master of the Order emeritus of Order of Preachers (Dominicans) (1558.05.29 – 1570.05.17) |
| 1583.03.02 | Cardinal-Bishop of Porto e Santa Rufina (1580.12.05 – 1583.03.02) Bishop emeritus of Perugia (Italy) (1564.09.06 – 1574.05.05) |
| 1583.08.22 | Cardinal-Priest of S. Callisto (1570.06.09 – 1583.08.22) Metropolitan Archbishop emeritus of Chieti (Italy) (1553.07.14 – 1568.01.14) Datary of His Holiness emeritus of Apostolic Dataria (1566 – 1570) |
| 1583.11.24 | |
| 1583.12.19 | Cardinal-Priest of S. Anastasia (1579.08.17 – 1583.12.19) |
| 1584.05.10 | Cardinal-Priest of S. Marco (1570.06.09 – 1584.05.10) Metropolitan Archbishop emeritus of Zadar (Croatia) (1566 – 1567) Camerlengo of the Holy Roman Church of Reverend Apostolic Camera (1570.05.10 – 1584.05.10) |
| 1584.06.14 | Cardinal-Priest of S. Pudenziana (1580.08.24 – 1584.06.14) Metropolitan Archbishop of Besançon (France) (1543.06.27 – 1584.06.14) |
| 1584.11.03 | Cardinal-Priest of S. Prassede (1564.11.17 – 1584.11.03) Metropolitan Archbishop of Milano (Italy) (1564.05.12 – 1584.11.03) Secretary of State of Apostolic Secretariat (1560 – 1584.11.03) |
| 1584.12.26 | Cardinal-Priest of S. Marco (1584.05.14 – 1584.12.26) Bishop emeritus of Kefalonia–Zakynthos (Greece) (1555.10.25 – 1560) |
| 1585.05.01 | Cardinal-Priest of S. Eustachio pro hac vice Title (1552.03.09 – 1585.05.01) Metropolitan Archbishop emeritus of Conza (Italy) (1539.08.08 – 1546.05.05) Camerlengo emeritus of Sacred College of Cardinals (1577.01.06 – 1578.01.08) |
| 1585.05.16 | Cardinal-Deacon of Ss. Vito e Modesto in Macello Martyrum (1566.03.06 – 1585.05.16) Commendatory Abbot emeritus of Nonantola (Italy) (1573 – 1582) |
| 1585.05.17 | Bishop emeritus of Massa Marittima e Populonia (Italy) (1579.04.27 – 1583) |
| 1585.07.10 | Cardinal-Priest of S. Nicola in Carcere pro hac vice Title (1562.07.06 – 1585.07.10) |
| 1585.07.18 | Cardinal-Priest of S. Maria in Ara Coeli (1578.03.03 – 1585.07.18) Patriarch of Alexandria (1570.11.08 – 1585.07.18) |
| 1585.10.06 | Cardinal-Priest of S. Lorenzo in Panisperna (1565.10.26 – 1585.10.06) Bishop emeritus of Squillace (Italy) (1568.02.27 – 1573.05.29) Librarian of Vatican Apostolic Library (1572.03.18 – 1585.10.06) |
| 1585.11.29 | Cardinal-Priest of S. Stefano al Monte Celio (1584.01.09 – 1585.11.29) |
| 1586.02.21 | |
| 1586.06.09 | Cardinal-Priest of S. Sisto (1572.06.16 – 1586.06.09) Prefect of Sacred Congregation of the Council (1580 – 1586.06.09) Major Penitentiary of Sacred Apostolic Penitentiary (1579.08.07 – 1586.06.09) Archpriest of Papal Basilica of Saint Mary Major (1581 – 1586.06.09) |
| 1586.09.21 | Cardinal-Bishop of Sabina (1578.10.03 – 1586.09.21) Metropolitan Archbishop of Besançon (France) ([1584.06.25] 1584.11.14 – 1586.09.21) |
| 1586.09.29 | Cardinal-Priest of S. Anastasia (1584.05.28 – 1586.09.29) |
| 1586.12.30 | Cardinal-Deacon of S. Maria in Via Lata (1583.12.19 – 1586.12.30) |
| 1587.01.21 | Metropolitan Archbishop of Kalocsa (Hungary) (1582.04.30 – 1587.01.21) |
| 1587.03.23 | Cardinal-Priest of S. Eufemia (1570.11.20 – 1587.03.23) |
| 1587.05.05 | Cardinal-Bishop of Palestrina (1583.03.04 – 1587.05.05) Bishop emeritus of Viterbo (Italy) (1566.10.07 – 1576?) Bishop emeritus of Tuscanella (Italy) (1566.10.07 – 1576?) |
| 1587.08.17 | Cardinal-Deacon of S. Eustachio (1587.01.07 – 1587.08.17) Commendatory Abbot of Nonantola (Italy) (1582 – 1587.08.17) Camerlengo of the Holy Roman Church of Reverend Apostolic Camera (1584.05.14 – 1587.08.17) |
| 1587.10.09 | Cardinal-Priest of S. Matteo in Merulana (1586.01.15 – 1587.10.09) Bishop of Cervia (Italy) (1585.11.15 – 1587.10.09) Archpriest of Papal Basilica of Saint Mary Major (1586.06? – 1587.10.09) |
| 1587.10.30 | Cardinal-Priest of SS. Trinità al Monte Pincio (1587.04.20 – 1587.10.30) |
| 1587.12.05 | Cardinal-Bishop of Porto e Santa Rufina (1583.03.09 – 1587.12.05) Grand Inquisitor of Commission of Roman and Universal Inquisition (1577 – 1587.12.05) |
| 1588.12.24 | Metropolitan Archbishop of Reims (France) (1574 – 1588.12.24) |
| 1589.01.02 | |
| 1589.03.02 | Cardinal-Bishop of Ostia–Velletri (1580.12.05 – 1589.03.02) Cardinal-Priest of S. Lorenzo in Damaso in commendum (1564.05.12 – 1589.03.02) Vice-Chancellor of Apostolic Chancery (1535.08.13 – 1589.03.02) Archpriest of Papal Basilica of Saint Peter (1543 – 1589.03.02) |
| 1589.10.02 | Cardinal-Bishop of Albano (1589.03.02 – 1589.10.02) |
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