| Date | Country | Change |
| 1965 | Diocese of Osnabrück lost territory to Diocese of Hildesheim | |
| 1965 | Diocese of Tyr (Maronite Rite) was promoted as Archdiocese of Tyr | |
| 1965.01.11 | Apostolic Prefecture of Bhagalpur was promoted as Diocese of Bhagalpur (became suffragan of Calcutta) | |
| 1965.01.26 | Diocese of Barra do Piraí was renamed as Diocese of Barra do Piraí–Volta Redonda | |
| 1965.02.02 | Diocese of Mondovì lost territory to Diocese of Albenga | |
| 1965.02.08 | Papal See of Roma lost territory to Diocese of Porto e Santa Rufina | |
| 1965.03.17 | Diocese of Tulcán was established (from Diocese of Ibarra and became suffragan of Quito) | |
| 1965.03.22 | Diocese of Chikwawa was established (from Metropolitan Archdiocese of Blantyre and became suffragan of Blantyre) | |
| 1965.03.22 | Diocese of Wonju 원주 was established (from Diocese of Chunchon 춘천 and became suffragan of Seoul 서울) | |
| 1965.03.22 | Apostolic Vicariate of Nakhorn-Rajasima was established (from Apostolic Vicariate of Ubon) | |
| 1965.03.22 | Diocese of Moroto was established (from Diocese of Gulu and became suffragan of Rubaga) | |
| 1965.05.05 | Diocese of Kabba was renamed as Diocese of Lokoja | |
| 1965.05.28 | Diocese of Arundel and Brighton was established (from Diocese of Southwark and became suffragan of Southwark) | |
| 1965.05.28 | Diocese of Southwark was promoted as Metropolitan Archdiocese of Southwark (with suffragan sees Arundel and Brighton, Plymouth and Portsmouth and lost territory to establish Diocese of Arundel and Brighton) | |
| 1965.06.14 | Diocese of Itabira was established (from Metropolitan Archdiocese of Diamantina and Metropolitan Archdiocese of Mariana and became suffragan of Mariana) | |
| 1965.06.24 | Apostolic Administration of Loreto was promoted as Territorial Prelature of Loreto (gained territory from Diocese of Recanati) | |
| 1965.06.24 | Diocese of Recanati lost territory to Territorial Prelature of Loreto | |
| 1965.07.06 | Apostolic Prefecture of Bafia was established (from Metropolitan Archdiocese of Yaoundé) | |
| 1965.07.06 | Apostolic Prefecture of Mendi was promoted as Apostolic Vicariate of Mendi | |
| 1965.07.06 | Apostolic Prefecture of Kaolack was promoted as Diocese of Kaolack (became suffragan of Dakar) | |
| 1965.07.06 | Apostolic Prefecture of Dapango was promoted as Diocese of Dapango (became suffragan of Lomé) | |
| 1965.07.10 | Diocese of Brownsville was restored (from Diocese of Corpus Christi and became suffragan of San Antonio) | |
| 1965.07.21 | Territorial Prelature of Calama was established (from Diocese of Antofagasta and Diocese of Iquique and became suffragan of La Serena) | |
| 1965.07.23 | Diocese of Barinas was established (from Metropolitan Archdiocese of Mérida and Diocese of Calabozo and became suffragan of Mérida) | |
| 1965.07.23 | Diocese of Cabimas was established (from Diocese of Maracaibo and became suffragan of Mérida) | |
| 1965.07.23 | Diocese of Los Teques was established (from Metropolitan Archdiocese of Caracas and became suffragan of Caracas) | |
| 1965.08.09 | Apostolic Vicariate of Río Muni was established (from Apostolic Vicariate of Fernando Poo) | |
| 1965.08.09 | Diocese of Hoima was established (from Metropolitan Archdiocese of Rubaga and Diocese of Fort Portal and became suffragan of Rubaga) | |
| 1965.08.31 | Ordinariate of Ordinariaat voor buitenlandse studenten in België was established | |
| 1965.10.12 | Archdiocese of Washington was promoted as Metropolitan Archdiocese of Washington (with suffragan see Virgin Islands) | |
| 1965.10.14 | Apostolic Vicariate of Guam was promoted as Diocese of Agaña (became suffragan of San Francisco) | |
| 1965.10.14 | Apostolic Prefecture of Western Transvaal was established (from Diocese of Johannesburg) | |
| 1965.10.14 | Diocese of Phú Cuong was established (from Metropolitan Archdiocese of Saigon and became suffragan of Saigon) | |
| 1965.10.14 | Diocese of Xuân Lôc was established (from Metropolitan Archdiocese of Saigon and became suffragan of Saigon) | |
| 1965.10.25 | Diocese of Ascoli Piceno lost territory to Diocese of Rieti | |
| 1965.11.17 | Diocese of Ascoli Piceno exchanged territory with Diocese of Teramo | |
| 1965.11.17 | Diocese of Teramo exchanged territory with Diocese of Ascoli Piceno | |
| 1965.12.03 | Diocese of Cairo (Syriac Rite) was established (without ecclesiastical province) | |
| 1965.12.16 | Diocese of Guarapuava was established (from Diocese of Campo Mourão, Diocese of Ponta Grossa and Diocese of Toledo and became suffragan of Curitiba) | |
| 1965.12.18 | Diocese of Bambari was established (from Metropolitan Archdiocese of Bangui and became suffragan of Bangui) | |
| 1965.12.18 | Diocese of Nouakchott was established (from Apostolic Prefecture of Saint-Louis du Sénégal and without ecclesiastical province) | |
| 1965.12.18 | Apostolic Vicariate of Bangkok was promoted as Metropolitan Archdiocese of Bangkok (with suffragan sees Bangnokhuek, Chantaburi and Chieng-Mai) | |
| 1965.12.18 | Apostolic Vicariate of Rajaburi was promoted as Diocese of Bangnokhuek (became suffragan of Bangkok) | |
| 1965.12.18 | Apostolic Vicariate of Chantaburi was promoted as Diocese of Chantaburi (became suffragan of Bangkok) | |
| 1965.12.18 | Apostolic Prefecture of Chieng-Mai was promoted as Diocese of Chieng-Mai (became suffragan of Bangkok) | |
| 1965.12.18 | Apostolic Vicariate of Nakhorn-Rajasima was promoted as Diocese of Nakhorn-Rajasima (became suffragan of Thare and Nonseng) | |
| 1965.12.18 | Apostolic Vicariate of Thare and Nonseng was promoted as Metropolitan Archdiocese of Thare and Nonseng (with suffragan sees Nakhorn-Rajasima, Ubon and Udonthani) | |
| 1965.12.18 | Apostolic Vicariate of Ubon was promoted as Diocese of Ubon (became suffragan of Thare and Nonseng) | |
| 1965.12.18 | Apostolic Prefecture of Udonthani was promoted as Diocese of Udonthani (became suffragan of Thare and Nonseng) |
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